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Get PriceJun 19, 2014 P. putida strains were grown in a defined M9 mineral medium consisting of per liter 12.8 g Na 2 HPO 4. 7H 2 O, 3 g KH 2 O 4, 1 g NH 4 Cl, and 0.5 g NaCl. This basic solution was autoclaved and subsequently supplemented with 0.12 g of MgSO 4. H 2 O and a trace element solution made of: 6.0 mg/L FeSO 4. 7H 2 O, 2.7 mg/L CaCO 3, 2.0 mg/L ZnSO 4
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Get PriceJan 21, 2020 In order to test the feasibility of E. coli to grow on ethanol, these strains were cultured in M9 mineral media with reduced glucose concentration of 1 g L 1. After the initial glucose was exhausted (about 8 h for strain BL21(DE3) and 9 h for the other three strains), 5 g L 1 of ethanol was added to the fermentation broth
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Get PriceDec 07, 2020 M9 medium, which contains 3.4 g/L Na 2 HPO12H 2 O, 0.6 g/L KH 2 PO 4, 0.1 g/L NaCl, 0.2 g/L NH 4 Cl supplemented with 20 g/L glucose, 50 mg/L tryptophan and 200 mM MOPS, was used for assessing growth of relevant strains. For lipopeptide surfactin production, B. amyloliquefaciens was incubated at 30 C and 180 rpm for 48 h in Landy medium
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Get PriceMay 03, 2016 Table 1 Product concentration and maximal conversion achieved in batch biotransformations for the production of geranyl glucoside at standard conditions (M9 mineral medium, pH 7.0, 0.2 g L 1 geraniol, 20 h, E. coli VvGT14a with 4.3 g L 1 dry cell mass) and at improved reaction conditions by the addition of the IL (HPYR)(NTF) as second
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